Cyanine based ratio fluorescent probe and its application in hypochlorite detection.

Hypochlorite (ClO-), a weakly acidic reactive oxygen species, plays a crucial role in antibacterial and anti-inflammatory defense mechanisms. However, elevated levels of ClO- or disruptions in endogenous sites can lead to tissue damage and various diseases including cardiovascular disease, neuronal degeneration, and arthritis. To address this, the development of a specific fluorescent probe with a built-in self-calibration ratio mode for the analysis and biological imaging of ClO- is essential. In this study, a cyanine-based fluorescent probe (Cy-H) was designed for ratiometric fluorescent detection of ClO-, utilizing its aggregation behavior as a novel approach in this field. Upon exposure to ClO-, the phenolic hydroxyl group in probe Cy-H was oxidized into benzoquinone, leading to the formation of cyanine products that displayed a strong tendency to aggregate. As a result, the maximum emission peak of the probe shifted from 700 nm to 485 nm. Notably, a linear relationship was observed between the peak intensity ratio (I485/I700) and the concentration of hypochlorite, with a limit of detection (LOD) of 0.49 µM. Furthermore, this probe was successfully employed for imaging analysis of hypochlorite in living cells and zebrafish.

A FRET Based Composite Sensing Material Based on UCNPs and Rhodamine Derivative for Highly Sensitive and Selective Detection of Fe3.

The existence of excessive concentration of iron ion (Fe3+) in water will do harm to the environment and biology. Presently, sensitive and selective determination of Fe3+ directly in real environment samples is still a challenging job because of the high complexity of the sample matrix. In this work, we reported a new sensor system for Fe3+ based on fluorescence resonance energy transfer (FRET) from upconversion nanoparticles (UCNPs) to Rhodamine derivative probe (RhB). The NaYF4: Yb, Er@SiO2@P(NIPAM-co-RhB) nanocomposites was constructed, in which PNIPAm was used as the probe carrier. The nanocomposites can not only be excited by infrared light to avoid the interference of background light in the Fe3+ detection process, but also enhance the detection signal output through temperature control. Under the optimum conditions, the RSD (Relative standard deviation) of actual sample measurements ranges was from 1.95% to 4.96%, with the recovery rate from 97.4% to 103.3%, which showed high reliability for Fe3+ detection. This work could be extended to sensing other target ions or molecules and may promote the widespread use of FRET technique.

Design and Synthesis of Novel Fluorescent Probe Based on Cyanobiphenyl and its Application in Detection of Hypochlorite.

Hypochlorite is an important biological reactive oxygen species, which plays a pivotal role in various life activities. Excessive presence in the human body or excessive intake in life causes a series of diseases. To monitor the hypochlorite level in living cells, organisms and environment water samples, we herein designed and synthesized three organic small molecule fluorescent probes with different recognition sites based on nitrile biphenyl. Through performance comparison, it was found that probe A-HM exhibited the best detection performance for hypochlorite with a low detection limit of 2.47 × 10-6 M. The introduction of hypochlorite will induce probe fluorescence A-HM to turn on, and the fluorescence colour will change from colourless to green. The application of A-HM in biological systems has been demonstrated by the imaging monitoring of hypochlorite in MCF-7, L929 cells and zebrafish. Furthermore, A-HM was also used for the accurate determination of the hypochlorite level in real water samples with high sensitivity and good recoveries.

One-step curing process of conductive paste based on a UV pulse laser for a frequency selective surface.

A frequency selective surface (FSS) is a two-dimensional periodic array structure, in which a common structure is composed of conductive paste and metal film. An ultraviolet (UV) pulse-laser-induced curing of conductive paste towards FSS was used to simultaneously realize the efficient curing of conductive paste and the removal of surplus materials. Through simple defocus control of the UV pulse laser when irradiating the workpiece, this technology was capable of reducing the energy density and increasing the irradiation range, thus preventing the conductive paste from being ablated and achieving efficient curing. In this context, the curing process of UV curable conductive paste was systematically studied through the Ohmic resistance and bonding force of the cured conductive paste. The curing effects of various conductive paste thicknesses and different laser scanning times at a certain laser scanning speed were also analyzed. The results showed that, after a controllable defocusing treatment, the UV pulse laser could effectively solidify the conductive paste and realize the electrical connection of materials on both sides of the pattern. The peel strength of the conductive paste was greater than 7.1 N/cm. However, the pulse laser curing method needs a longer curing time when compared with the continuous UV curing method.

Flow-induced translocation of vesicles through a narrow pore.

We use finite element method to investigate the flow-induced translocation of vesicles through a narrow pore from a dynamic point of view. In order to complete the coupling between fluid flow and the vesicle membranes, we employ the fluid-structure interactions with the arbitrary Lagrangian-Eulerian method. Our results demonstrate that the vesicle shows similar shape change from bullet-like to dumbbell-like, dumbbell-like to bulb-like, and bulb-like to parachute-like if it is pushed by flow field to pass through a narrow pore smaller than its size. We further find that the strain energy exhibits a higher peak and a lower peak in the whole translocation process, where the higher peak corresponds to the dumbbell-like shape and the lower peak corresponds to the parachute-like shape due to more stretching of the membrane for the dumbbell-like shape than that of the parachute-like shape. The translocation time of the vesicle from one side to the other side of the narrow pore decreases with the increase of inlet velocity, but the strain energy exhibits an increase, which implies that the vesicle needs more time to complete the translocation with the lower inlet velocity, but the requirement for the mechanical properties of the membrane is lower. Our work answers the mapping between the positions of the vesicles and deformed states with the stress distribution and change of strain energy, which can provide helpful information on the utilization of vesicles in pharmaceutical, chemical, and physiological processes.

An investigation on pollutant emissions from co-firing of RDF and coal.

Pollutant emissions from co-firing of refuse derived fuel (RDF) and coal were investigated in a vortexing fluidized bed combustor (VFBC). RDF-5 was made of common municipal solid waste (MSW). CaCO(3) was injected in the combustor to absorb HCl at 850 degrees C. The results show that NO(x) and HCl emissions increase with RDF-5 co-firing ratio. The NO(x) concentration in flue gas at the bottom of the combustor is higher than that at the top. However, the trend of HCl released is reverse compared with NO(x) emissions. It was found that the HCl concentration decreases with increasing the molar ratio of Ca/Cl. However, the effect of CaCO(3) addition on HCl retention is not significant when the molar ratio of Ca/Cl is higher than 5. The chlorine content in fly ash increases obviously with the molar ratio of Ca/Cl. PCDD/Fs emissions decrease slightly with an addition of CaCO(3). In this study incomplete combustion is regarded as the main cause for polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) formation.